The research was published by researchers at the Southwestern Medical Center at the University of Texas.
This study shows that using an enzyme substitute from Prevotella and Francisella 1 (Cpf1), a commonly used gene-editing CRISPR-associated protein 9 (Cas9), can correct disease-related lesions in animal models as well as in human cells.
The study, published in the journal Science Advances, may have a breakthrough in the treatment of Duchenne muscular dystrophy.
In the article, the University of Texas Southwest team used induced pluripotent stem cells (iPS) from DMD patient fibrocytes to disrupt early stop codons in exon 51 using CRISPR-Cpf1.
(Web picture)
Exon 51 results in a loss of dystrophin expression in approximately 13% of patients.
This gene editing technique repairs the expression of dystrophin in induced pluripotent stem cells (iPS) and cardiomyocytes from cells and increases the contractility of cardiomyocytes.
Cpf1 first discovered that Zhang Feng, a team at the Massachusetts Institute of Technology and the Harvard University Institute, identified the relevant endonuclease as a CRISPR. Since Cpf1 is smaller than Cas9, it is easier to be loaded into a vehicle with potential for different gene sequences.
In December last year, Editas Pharmaceuticals, which specializes in gene editing therapy, licensed the intellectual property rights to use Cpf1 for CRISPR gene editing from the Broad Institute.
The drainage pump is often used for urban waterlogging, rural and forest fires to prevent and deal with emergencies. It supplies water to the fire site through pressure pipelines or fire monitors, and can also be used for drainage when large-scale flooding occurs.
When there is a considerable distance between the ponding point and the drainage site, the supplementary pipe can be used to extend to 1000m to realize long-distance drainage. When there is water, it can replace the fire truck and be used as the pump station. Equipped with hydraulic bit, it can extinguish peat fire and also can use fire monitor.
Flood Pump,Water Pump Factory,Flood Pump Supplier,Flood Control Pump
Denilco Environmental technology(Suzhou)Co., Ltd. , https://www.wflood.com