Introduction to the working principle of the refrigerated centrifuge

The working principle of the refrigerated centrifuge.

Centrifugation is the use of a powerful centrifugal force generated by the high-speed rotation of the refrigerated centrifuge rotor to accelerate the sedimentation velocity of the particles in the liquid, thus leaving the different sedimentation coefficients and buoyant density of the sample. Therefore, the refrigerated centrifuge mainly extracts the required components from the liquid mixture. According to the different densities of each substance, the high-density liquid is suspended at the bottom layer, and the dense liquid is suspended on the bottom layer, so that the liquid is layered. , extract the pure things we need. Generally speaking, refrigerated centrifuges are mainly used in many fields such as medicine and chemical industry.

So, what is the working principle of the refrigerated centrifuge? Hunan Hengnuo Equipment Co., Ltd. mentioned that when the floating liquid containing fine particles is still standing, the suspended particles gradually sink due to the action of the gravity field. If the particles are heavier, the sinking speed will be faster. If the density is smaller, the liquid particles will float, and the speed at which the particles move under the gravitational field is closely related to the size, shape and density of the particles, and also related to the strength of the gravity field and the viscosity of the liquid, like the size of red blood cells. Particles, a few micrometers in diameter, can be observed under normal gravity.

During the operation of the refrigerated centrifuge, when the substance settles in the medium, it is accompanied by a diffusion phenomenon, and the diffusion is unconditionally absolute. Diffusion is inversely proportional to the mass of the material. The smaller the particle, the more severe the diffusion, and the sedimentation is relative. If it is conditional, it must be subjected to external force to move. The sedimentation is proportional to the weight of the object. The larger the particle, the faster the sedimentation. For a few micrometers of particles such as viruses or proteins, they are in a colloidal or semi-colloidal state in solution. It is impossible to observe the sedimentation process by gravity alone. The smaller the particles, the slower the sedimentation and the more severe the diffusion phenomenon, so it is necessary to use a centrifuge to generate a strong centrifugal force to force the particles to overcome the diffusion and produce sedimentation motion.

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