Key technical description of artificial insemination in cattle

Estrus identification of early estrus, cows loss of appetite, love to cry, frequent urination, excitement. The vagina began to flow mucus, mucous membrane congestion, pale red, slightly swollen cervix, thin and transparent mucus. During the estrus season, the vulva swells obviously. From time to time, it lifts its head and looks forward. It lifts its back and raises its tail. It accepts the bulls to climb. The vagina film is flush and shiny. The cervix is ​​congested brightly, swollen and open. The mucus volume is large and the traction is strong. Transparent and non-tacky During late estrus, the vulva swelled and swollen. The thick mucus appeared on the pudendal skin or on the tail of the root. The degree of hyperemia of the vaginal mucous membranes faded. It was dark red, and the mucus was less thick and thick. It was translucent or milky white. Sticky, cervical swelling and neck opening have contracted. Cattle often mixed with bloodshot efflux. During ovulation, the libido disappeared completely, the bull did not follow, the feeding was normal, the vaginal mucous membrane was pink, the cervix was closed, the mucus was small, and the milky skin was thick. In general, late-season breeding is ideal.
The time of artificial insemination and breeding of yellow cattle during breeding time is suitable in the late stage of estrus. In simple terms, if estrus is found in the morning, the morning cows will be inseminated at the breeding station; if the estrus is in the afternoon, the morning cows will be inseminated at the breeding station the next morning. If only one insemination is performed, it can be performed 8-12 hours after the refusal to climb. If the second insemination method is applied, the cow will receive a climb in the morning, insemination once in the afternoon, and once in the second day; the cow will receive a climb in the afternoon and be inseminated once in the afternoon and the following day. A love period loses twice and the effect is better.
The equipment is prepared with a straw infusion gun and a thermometer, one for the scorpions, and one for the thin tubes, one for the nail scissors, and one for the thermostatic water bath.
Fine tube freeze-thawing temperature bath will be adjusted to the temperature of 40 °C -42 °C, with tweezers from the liquid nitrogen tank to remove the fine tube of frozen essence, can not pull the thin tube bag out of the liquid nitrogen tank, the tube into the water bath Shake it for 10-15 seconds until it warms up evenly. Then microscopic examination, with a small amount of semen slides in 150 times under the microscope, to 0.3 or more qualified, with a good absorbent paper towel to dry the thin tube, check the semen type and model to take, with a thin tube tampon The end is at the bottom, raise the hand flush with the eye, cut the closed end of about 0.5 centimeters, cut the mouth to be positive, the cross section should be flush, cut a complete circular incision, and then put into the insemination gun for use.
Rectal grasping method insemination
1, remove the rectum Yu feces sperm will be left hand nails cut short, flat, clean the palm of hand disinfection, and coated with soapy water, the fingers into a cone, slowly rotating into the anus, rectal rectum feces.
2, grasp the cervix will extend his left hand into the anus, hand support spread palm down, press strokes, across the straight field in the bottom of the pelvis can touch a long round, hard texture of the stick, this is the cervix, will son The back of the cervix is ​​lightly fixed in the hand.
3, insemination is 45 degrees upward into the insemination gun gradually twisted infusion gun, then moved horizontally along the vaginal wall, so you can avoid entering the urethra and bladder, continue to move the infusion gun forward until the tip of the gun arrives Cervical mouth. The two hands coordinate with each other to adjust the direction, so that the insemination gun slowly passes through the crumpling wall of the cervical canal and enters the deep cervical or uterine body. The hand holding the cervix is ​​held and the gun plug is fully pushed into the barrel. Special attention is paid to the insemination. Do not blindly insert the gun too deeply. Under no circumstances should semen be sent beyond the curvature of the uterine horn, so as not to affect sperm capacitation and damage the endometrium and affect fertilization. When the follicle development side cannot be confirmed, blindly sending the semen too deep may cause the ovulation side to have no sperm so that the egg cannot be fertilized, resulting in failure of the breeding.

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