Adjustment of the Olympus microscope illumination light path system:
In order to make the Olympus microscope's field of view evenly and fully illuminated, it is necessary to adjust the illumination path system when the Olympus microscope is first installed and debugged. This is the correct use of the Olympus microscope. Obtain the correct and achievable means and the most basic requirements. In addition, the correct adjustment of the illumination light path system is a necessary step after replacing the light source bulb in the Olympus microscope process, and is also a necessary means to test the performance of the Olympus microscope from time to time during daily use.
The adjustment of the Olympus microscope illumination light path system has the following four main contents :
(1) Preliminary adjustment of the light source lamp room outside the microscope
1 First open the outer casing of the lamp chamber, press the spring clip to insert the halogen bulb into the socket. Avoid direct contact with the bulb when the device is installed (can be separated by a soft cloth or paper), so as to avoid fingerprints and other dirt on the bulb, affecting the bulb. The service life.
2 Place the lamp room on the table. After turning on the power, use a special screwdriver to adjust the focus knob hole of the lamp (labeled "â†â†’"), so that the filament is projected on the wall 1-2m away, and the filament is imaged. To clear; then adjust the height of the lamp to adjust the wire hole (marked "-"), so that the filament position is appropriate; then adjust the left and right position of the lamp to adjust the screw hole (marked with "-"), so that the left and right positions of the filament are suitable .
1 First open the outer casing of the lamp chamber, press the spring clip to insert the halogen bulb into the socket. Avoid direct contact with the bulb when the device is installed (can be separated by a soft cloth or paper), so as to avoid fingerprints and other dirt on the bulb, affecting the bulb. The service life.
2 Place the lamp room on the table. After turning on the power, use a special screwdriver to adjust the focus knob hole of the lamp (labeled "â†â†’"), so that the filament is projected on the wall 1-2m away, and the filament is imaged. To clear; then adjust the height of the lamp to adjust the wire hole (marked "-"), so that the filament position is appropriate; then adjust the left and right position of the lamp to adjust the screw hole (marked with "-"), so that the left and right positions of the filament are suitable .
(2) Inspection and correction of the position of the light source illuminator ( filament ) in the Olympus microscope:
The purpose is to adjust the image end of the illuminant into the field of view of the objective lens to ensure sufficient and uniform illumination of the Olympus microscope from the perspective of the light source. This is to adjust the Kule illumination system. Prerequisites. The basic tools needed: equipped with the Olympus microscope for the telescope.
1 Unplug the frosted glass sleeve in the lamp library and replace the lamp chamber on the Olympus microscope;
2 Select 10× objective lens, open the light source program to find the sample and adjust the focus clearly, then use 40× objective lens to adjust the sample clearly (40× objective lens can see the whole picture of the filament);
3 Open the aperture stop and field diaphragm of the condenser to the maximum;
4 Unplug one of the eyepieces, replace the centering telescope, grab the white part, and flex the black eyepiece with the other hand to see the filament image in the field of view;
5 If the position of the filament is not suitable, adjust the “──†hole, adjust the filament image in the horizontal direction, adjust the “──†hole, and adjust the wire image in the vertical direction until the filament image is adjusted to just fill the aperture of the objective lens. Light round image
6 After the adjustment is completed, insert the frosted glass sleeve back into the original position, unplug the centering telescope, and change back to the eyepiece for the next adjustment. The above adjustment of the illumination source lamp chamber outside the Olympus microscope and the calibration of the position of the light source illuminator in the Olympus microscope are only required when the Olympus microscope is first installed and debugged and the bulb is replaced. When using an Olympus microscope, you can't arbitrarily adjust it. In case of confusion, you can return to the original state by following the above steps.
The purpose is to adjust the image end of the illuminant into the field of view of the objective lens to ensure sufficient and uniform illumination of the Olympus microscope from the perspective of the light source. This is to adjust the Kule illumination system. Prerequisites. The basic tools needed: equipped with the Olympus microscope for the telescope.
1 Unplug the frosted glass sleeve in the lamp library and replace the lamp chamber on the Olympus microscope;
2 Select 10× objective lens, open the light source program to find the sample and adjust the focus clearly, then use 40× objective lens to adjust the sample clearly (40× objective lens can see the whole picture of the filament);
3 Open the aperture stop and field diaphragm of the condenser to the maximum;
4 Unplug one of the eyepieces, replace the centering telescope, grab the white part, and flex the black eyepiece with the other hand to see the filament image in the field of view;
5 If the position of the filament is not suitable, adjust the “──†hole, adjust the filament image in the horizontal direction, adjust the “──†hole, and adjust the wire image in the vertical direction until the filament image is adjusted to just fill the aperture of the objective lens. Light round image
6 After the adjustment is completed, insert the frosted glass sleeve back into the original position, unplug the centering telescope, and change back to the eyepiece for the next adjustment. The above adjustment of the illumination source lamp chamber outside the Olympus microscope and the calibration of the position of the light source illuminator in the Olympus microscope are only required when the Olympus microscope is first installed and debugged and the bulb is replaced. When using an Olympus microscope, you can't arbitrarily adjust it. In case of confusion, you can return to the original state by following the above steps.
(3) adjusting the correct lighting Culler (of Kohler) system:
One of the main tasks of the correct commissioning of the Olympus microscope is the adjustment of the illumination optical system, and the key to this is the adjustment of the Kühler lighting system. For every person who uses Olympus microscopes, especially those who are used for photomicrography, they should have a certain understanding and mastery of the principles and adjustment steps of the Kühler lighting system in order to give full play to Olympa. The functions that the microscope should have, the photos taken can be more consistent and perfect in effect. The principle of the Kühler illumination system is simply that the light emitted from any point on the source illuminator can illuminate the field of view of the Olympus microscope, and the light emitted by each point on the source illuminator is collected in Olin. Very adequate and uniform illumination is achieved in the field of view of the Bass microscope. The purpose of adjusting the Kühler illumination system is to achieve uniform and sufficient illumination of the observed field of view to prevent stray light from affecting or interfering with the image system to avoid fogging on the film during photography. High-adjustment of the necessary components of the Kühler lighting system: field diaphragm, concentrating mirror system for axial adjustment.
1 Use 10× objective lens and 10× eyepiece;
2 Put the front lens of the condenser into the optical path, adjust the aperture stop to a moderate position (not too big or small), then raise the condenser to the top position, and adjust the condenser dial to the bright field "J" position;
3 adjust the field diaphragm to the minimum (0.1);
4 Place the biological sample that has been sealed on the stage, turn on the light source, and adjust the focus clearly;
5 There will be a partially illuminated area or bright spot in the field of view, which is a blurred image of the field of view, in which the details of the sample can be clearly seen; outside it is a darker view, not necessarily See the details of the sample clearly;
6 The concentrating mirror is slightly adjusted downwards, so that the bright spots in the field of view gradually become smaller and gradually become a clear polygonal image, which is a clear image of the field of view;
7 In general, the polygon image is not in the center of the field of view. You need to adjust the pair of centering screws of the condenser to adjust the image of the field diaphragm to the center position.
8 Gradually open the large field of view, make the polygon image become the inscribed polygon of the field of view, and further check the condition of the adjustment. If the alignment is not ideal, continue to fine-tune the centering screw;
9 The field diaphragm is slightly enlarged slightly, so that its polygon image just disappears on the edge of the field of view. At this point, the Kühler lighting system is adjusted. After the adjustment of the Kühler lighting system, the entire field of view is illuminated evenly, and the micrographs taken are bright and clear with normal contrast. Special attention should be paid during the future use: a. The field diaphragm cannot be opened arbitrarily, but the field diaphragm can be reduced as the objective lens magnification increases, and it becomes larger as the objective lens magnification decreases; b. The height of the concentrating mirror is not allowed to be adjusted, otherwise it will destroy the adjusted Kühler lighting system; c. When using an objective lens of 10× or less, the front lens of the condenser should be placed outside the optical path. When using an objective lens of 10× or more, the front lens should be placed into the optical path; d. Regarding the problem of matching the objective lens multiple with the field diaphragm pupil size, in the actual use process, it is not necessary to take small or open field of view diaphragm as a general observation, but in order to avoid stray light to the camera system Interference, so that you can take a more perfect picture, you should use the objective lens of each multiple to adjust the field diaphragm to just disappear on the side of the observed field of view. This is a complicated work, but it is not Do not. A relatively simple method is to adjust the field diaphragm corresponding to each of the multiple objective lenses in advance, and make a mark, and then directly adjust to the corresponding position according to the mark when using.
One of the main tasks of the correct commissioning of the Olympus microscope is the adjustment of the illumination optical system, and the key to this is the adjustment of the Kühler lighting system. For every person who uses Olympus microscopes, especially those who are used for photomicrography, they should have a certain understanding and mastery of the principles and adjustment steps of the Kühler lighting system in order to give full play to Olympa. The functions that the microscope should have, the photos taken can be more consistent and perfect in effect. The principle of the Kühler illumination system is simply that the light emitted from any point on the source illuminator can illuminate the field of view of the Olympus microscope, and the light emitted by each point on the source illuminator is collected in Olin. Very adequate and uniform illumination is achieved in the field of view of the Bass microscope. The purpose of adjusting the Kühler illumination system is to achieve uniform and sufficient illumination of the observed field of view to prevent stray light from affecting or interfering with the image system to avoid fogging on the film during photography. High-adjustment of the necessary components of the Kühler lighting system: field diaphragm, concentrating mirror system for axial adjustment.
1 Use 10× objective lens and 10× eyepiece;
2 Put the front lens of the condenser into the optical path, adjust the aperture stop to a moderate position (not too big or small), then raise the condenser to the top position, and adjust the condenser dial to the bright field "J" position;
3 adjust the field diaphragm to the minimum (0.1);
4 Place the biological sample that has been sealed on the stage, turn on the light source, and adjust the focus clearly;
5 There will be a partially illuminated area or bright spot in the field of view, which is a blurred image of the field of view, in which the details of the sample can be clearly seen; outside it is a darker view, not necessarily See the details of the sample clearly;
6 The concentrating mirror is slightly adjusted downwards, so that the bright spots in the field of view gradually become smaller and gradually become a clear polygonal image, which is a clear image of the field of view;
7 In general, the polygon image is not in the center of the field of view. You need to adjust the pair of centering screws of the condenser to adjust the image of the field diaphragm to the center position.
8 Gradually open the large field of view, make the polygon image become the inscribed polygon of the field of view, and further check the condition of the adjustment. If the alignment is not ideal, continue to fine-tune the centering screw;
9 The field diaphragm is slightly enlarged slightly, so that its polygon image just disappears on the edge of the field of view. At this point, the Kühler lighting system is adjusted. After the adjustment of the Kühler lighting system, the entire field of view is illuminated evenly, and the micrographs taken are bright and clear with normal contrast. Special attention should be paid during the future use: a. The field diaphragm cannot be opened arbitrarily, but the field diaphragm can be reduced as the objective lens magnification increases, and it becomes larger as the objective lens magnification decreases; b. The height of the concentrating mirror is not allowed to be adjusted, otherwise it will destroy the adjusted Kühler lighting system; c. When using an objective lens of 10× or less, the front lens of the condenser should be placed outside the optical path. When using an objective lens of 10× or more, the front lens should be placed into the optical path; d. Regarding the problem of matching the objective lens multiple with the field diaphragm pupil size, in the actual use process, it is not necessary to take small or open field of view diaphragm as a general observation, but in order to avoid stray light to the camera system Interference, so that you can take a more perfect picture, you should use the objective lens of each multiple to adjust the field diaphragm to just disappear on the side of the observed field of view. This is a complicated work, but it is not Do not. A relatively simple method is to adjust the field diaphragm corresponding to each of the multiple objective lenses in advance, and make a mark, and then directly adjust to the corresponding position according to the mark when using.
(4) Correct use of aperture stop:
Since the aperture stop of the condenser can affect the resolution of the Olympus microscope, the correct method of use should be used. In the past, due to insufficient understanding of the aperture stop, it was often used as a tool to adjust the brightness of the field of view. Although adjusting the aperture stop can change the brightness of the field of view to a certain extent, it will directly affect the contrast, contrast and resolution of the image, and should be avoided as much as possible during use. In order to play the role of the concentrating aperture of the concentrating mirror, in order to obtain the best resolution when observing, especially in photomicrography, it is necessary to adjust the aperture stop after the sample is sharply adjusted, when each objective lens is replaced by one. Make it exactly 2/3 of the numerical aperture of the objective lens (the aperture of the objective lens). The adjustment method is to focus on the black phase difference ring in the field of view with the centering telescope, adjust the aperture stop, and you can see a polygonal aperture stop image, and then adjust to 2/3 of the objective lens aperture image, that is, between the black phase difference rings. Between the outer and the circular view. For the sake of convenience, the aperture stop corresponding to each multiple objective lens can be pre-adjusted and marked so as not to be re-adjusted every time.
Since the aperture stop of the condenser can affect the resolution of the Olympus microscope, the correct method of use should be used. In the past, due to insufficient understanding of the aperture stop, it was often used as a tool to adjust the brightness of the field of view. Although adjusting the aperture stop can change the brightness of the field of view to a certain extent, it will directly affect the contrast, contrast and resolution of the image, and should be avoided as much as possible during use. In order to play the role of the concentrating aperture of the concentrating mirror, in order to obtain the best resolution when observing, especially in photomicrography, it is necessary to adjust the aperture stop after the sample is sharply adjusted, when each objective lens is replaced by one. Make it exactly 2/3 of the numerical aperture of the objective lens (the aperture of the objective lens). The adjustment method is to focus on the black phase difference ring in the field of view with the centering telescope, adjust the aperture stop, and you can see a polygonal aperture stop image, and then adjust to 2/3 of the objective lens aperture image, that is, between the black phase difference rings. Between the outer and the circular view. For the sake of convenience, the aperture stop corresponding to each multiple objective lens can be pre-adjusted and marked so as not to be re-adjusted every time.
Information link: Where to buy an Olympus microscope?
By cooperating with Olympus manufacturers, Beiden promised to sell the Olympus microscope 100% original authentic, discount sale. For details on Olympus microscopes, product specifications, usage methods, market prices, etc., please visit www. Vedeng. Com, or call the hotline 4006-999-556, we will provide you with free selection support for Olympus microscope.
For related Olympus microscope product information, click >>>>>>> Olympus microscope cx31 , Olympus microscope cx41
China Extract Powder For Use As Dietary Supplement Extract Powder, Extract Powder Manufacturer
Shaanxi Kang New Pharmaceutical co., Ltd. , https://www.apipepdites.com