Scientists achieve label-free microscopy of Alzheimer's amyloid deposits
November 21, 2018 Source: Xinhua News Agency Author: Chou Yi
Window._bd_share_config={ "common":{ "bdSnsKey":{ },"bdText":"","bdMini":"2","bdMiniList":false,"bdPic":"","bdStyle":" 0","bdSize":"16"},"share":{ }};with(document)0[(getElementsByTagName('head')[0]||body).appendChild(createElement('script')) .src='http://bdimg.share.baidu.com/static/api/js/share.js?v=89860593.js?cdnversion='+~(-new Date()/36e5)];The reporter recently learned from Fudan University that a study conducted by a group of Fudan University, Harvard University and the Massachusetts General Hospital has used stimulated Raman imaging technology to detect Alzheimer's amyloid deposits for the first time. This method is also expected to be used to explore other neurodegenerative diseases such as Parkinson's.
Alzheimer's disease is a neurodegenerative disease that causes dementia; it is hypothesized that the Aβ protein in the brain is misfolded, causing a reaction that eventually kills neurons and forms amyloid deposits. Therefore, the observation of amyloid precipitation has become the key to studying the disease mechanism.
The "Science·Progress" journal recently published the research results of the research team of the Department of Physics of Fudan University, Ji Minbiao and its cooperation team. The paper reveals a technological advance in the use of novel microscopic imaging techniques for label-free imaging of amyloid plaques with misfolded proteins. The Department of Physics of Fudan University is the first completion unit of the thesis.
In the past, to observe the protein precipitation formed in the brains of patients with Alzheimer's disease, it is usually necessary to add a label. Because the difference between protein precipitation and other substances is not clear, it is necessary to use dyes to make specific structures appear specific. colour. However, this method has significant disadvantages. Exogenous molecules such as fluorescent agents are inherently unstable, and not only change over time, but also affect the nature of the observed object and cause it to change.
According to Ji Minbiao, the research team used stimulated Raman scattering (SRS) microscopy to observe protein precipitation in Alzheimer's disease. Compared with the addition of fluorescer to observe protein precipitation, stimulated Raman scattering microscopy technology does not rely on the nature of the molecule itself, is not affected by photobleaching, and does not interfere with any foreign matter, and does not need to make samples. Some very complicated treatments are therefore more suitable for long-term in vivo observations.
To further validate the accuracy of the new technique, the team compared the images of stimulated Raman imaging with the addition of markers in the same tissue section, and the results showed that the detection ability of both amyloid plaques was almost the same. In addition, the research team also compared the results of stimulated Raman imaging and dye-labeled fluorescence imaging in fresh tissue, and found that the detection of plaque morphology was completely consistent.
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