SLEE cryostat working procedure
1. Turn on the main power switch, check/set the freezer temperature, cold stage temperature, sample head temperature, slice thickness, and enter the normal interface (generally. This machine is powered on for 24 hours, it is always on, so it only starts cold when going to work. Refrigeration in the station, cooling off the cold station when it is off work).
2. Slicing procedure - place the specimens that have been "taken" with the embedding agent on the sample head, set the frozen platform "quickly frozen", and lock the "sliced ​​handwheel". After a while. Fix the sample clip on the frozen specimen, first trim, then slice, and patch.
3. After the patch, it should be immersed in the "acetic acid alcohol solution" in time to "fix".
4. Cut samples are numbered, stored for later use or used in downstream processes.
5. Shutdown procedure - lock the handwheel, clean the freezer, open the freezer door, and cut off the power switch. This machine is a continuous working machine, it is continuously energized. Under normal circumstances, it will not be turned off. When it is not used for a short time, the cold stage cooling will be turned off. If the temperature of the freezer is set to above 0 °C, it will be deactivated.
SLEE cryostat considerations
1. Check before starting the machine, whether the installation of the instrument is stable and stable, whether the connection between the power plug and the socket is reliable and the grounding is good. There are certain gaps around to facilitate ventilation, heat dissipation and voltage.
2. Please pay attention to "biosafety protection" during operation. For specimens with infectious diseases, clean and disinfect the work in time, and lock the handwheel before placing/removing the specimen to prevent the blade from hurting hands and moving parts.
3. Prevent liquid from flowing into the electrical parts of the instrument, causing damage to the appliance. The buttons and knobs should be lightly touched and rotated. Pay attention to the power supply capacity of the socket, such as long-term low voltage operation. Will affect the normal operation of the machine. Pay attention to ventilation and cooling.
Common faults in SLEE cryostats
1. After two hours of power on (the preset temperature has been reached), during the operation of the machine, the preset temperature will not reach the preset temperature (for example, the preset temperature is -22 ° C, but at this temperature, the machine is running. After half an hour, the temperature inside the machine rises to -14 to 17 °C.)
Reason and analysis: Always switch the glass door when slicing. If this operation is not very fast, plus the room temperature is relatively high, the temperature inside the chassis is not easy to maintain the set temperature.
2. Does the machine have to be turned on continuously? If you shut down once in an experiment, will it cause more damage to the machine?
Reasons and analysis: It needs to be continuously turned on. If it is not used, it will keep the temperature at the low temperature. If it needs to be turned on again after the shutdown, it needs to stop for at least 12 hours before turning it on.
3. The handle does not correspond to the position of the feed.
Reason and analysis: The mechanical transmission part is misaligned; after adjusting the corresponding position, install the belt and tighten the positioning card, and add oil lubrication to the moving friction part.
4. Causes and analysis of cooling effect reduction: poor heat dissipation of the condensing radiator or serious frosting of the evaporator; first, cleaning the radiator and flushing with water; second, defrosting, defrosting every time, especially in summer, the humidity is very high. Frosting minimizes the number of door opening.
5. Reasons and analysis for non-refrigeration: refrigerant leakage; addition of refrigerant.
1. Turn on the main power switch, check/set the freezer temperature, cold stage temperature, sample head temperature, slice thickness, and enter the normal interface (generally. This machine is powered on for 24 hours, it is always on, so it only starts cold when going to work. Refrigeration in the station, cooling off the cold station when it is off work).
2. Slicing procedure - place the specimens that have been "taken" with the embedding agent on the sample head, set the frozen platform "quickly frozen", and lock the "sliced ​​handwheel". After a while. Fix the sample clip on the frozen specimen, first trim, then slice, and patch.
3. After the patch, it should be immersed in the "acetic acid alcohol solution" in time to "fix".
4. Cut samples are numbered, stored for later use or used in downstream processes.
5. Shutdown procedure - lock the handwheel, clean the freezer, open the freezer door, and cut off the power switch. This machine is a continuous working machine, it is continuously energized. Under normal circumstances, it will not be turned off. When it is not used for a short time, the cold stage cooling will be turned off. If the temperature of the freezer is set to above 0 °C, it will be deactivated.
SLEE cryostat considerations
1. Check before starting the machine, whether the installation of the instrument is stable and stable, whether the connection between the power plug and the socket is reliable and the grounding is good. There are certain gaps around to facilitate ventilation, heat dissipation and voltage.
2. Please pay attention to "biosafety protection" during operation. For specimens with infectious diseases, clean and disinfect the work in time, and lock the handwheel before placing/removing the specimen to prevent the blade from hurting hands and moving parts.
3. Prevent liquid from flowing into the electrical parts of the instrument, causing damage to the appliance. The buttons and knobs should be lightly touched and rotated. Pay attention to the power supply capacity of the socket, such as long-term low voltage operation. Will affect the normal operation of the machine. Pay attention to ventilation and cooling.
Common faults in SLEE cryostats
1. After two hours of power on (the preset temperature has been reached), during the operation of the machine, the preset temperature will not reach the preset temperature (for example, the preset temperature is -22 ° C, but at this temperature, the machine is running. After half an hour, the temperature inside the machine rises to -14 to 17 °C.)
Reason and analysis: Always switch the glass door when slicing. If this operation is not very fast, plus the room temperature is relatively high, the temperature inside the chassis is not easy to maintain the set temperature.
2. Does the machine have to be turned on continuously? If you shut down once in an experiment, will it cause more damage to the machine?
Reasons and analysis: It needs to be continuously turned on. If it is not used, it will keep the temperature at the low temperature. If it needs to be turned on again after the shutdown, it needs to stop for at least 12 hours before turning it on.
3. The handle does not correspond to the position of the feed.
Reason and analysis: The mechanical transmission part is misaligned; after adjusting the corresponding position, install the belt and tighten the positioning card, and add oil lubrication to the moving friction part.
4. Causes and analysis of cooling effect reduction: poor heat dissipation of the condensing radiator or serious frosting of the evaporator; first, cleaning the radiator and flushing with water; second, defrosting, defrosting every time, especially in summer, the humidity is very high. Frosting minimizes the number of door opening.
5. Reasons and analysis for non-refrigeration: refrigerant leakage; addition of refrigerant.
High-Transparency Net,Round Wire Shade Net,Round Thread Shade Net,Uv Plastic Sun Shade Net
Changzhou Green Nets Co.,Ltd. , https://www.czglnets.com