Commonly used internal reference antibody and tag antibody

Anti-β-Tubulin, Anti-β-Actin, Anti-GAPDH, Anti-GFP Tag, Anti-RFP Tag, etc.

Epitope Biotech Inc. custom-made high-quality mouse monoclonal antibodies specific for specific epitopes, with the highest sensitivity and highest specificity, and cost-effective compared to similar products produced by other companies. Products include: tag antibodies, internal references (loading controls), and antibodies for signal transduction studies.

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Figure 1: EBI's anti-His tag monoclonal antibody (Anti-His Tag (HIS.H8) mAb) is the world's most popular His-tag monoclonal antibody. We estimate that thousands of laboratories are using this antibody. Several articles published in the top science journals using this antibody fully demonstrate the high sensitivity and high affinity of this antibody.

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Figure 2: EBI currently offers a range of commonly used internal reference (loading controls) monoclonal antibodies. The picture shows the results of hybridization of three 10 ug/lane internal reference monoclonal antibodies in a mouse brain tissue lysate. The 50 kD band is a band obtained by hybridization of anti-beta-Tubulin (BT7R) antibody at 1:2000 dilution (0.5 ug/ml); the 42 kD band is anti-beta-actin (Anti a band obtained by hybridization of -β-Actin (BA3R)) antibody at 1:1000 (1 ug/ml); a 37 kD band is anti-GAPDH (Anti-GAPDH (GA1R)) antibody at 1:5000 (0.2 ug/ml) The resulting bands are crossed.

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Figure 3: EBI's anti-GAPDH internal reference mAb (Anti-GAPDH (GA1R) loading control mAb) is excellent, with a 1:100,000 dilution of the antibody for protein hybridization to detect clear bands. It is the only antibody on the market that recognizes the following GAPDH proteins: E. coli BL-21, insect cell Sf9, yeast Saccharomyces cerevisiae, in addition to humans, mice, rats, rabbits, chickens, hamsters, and more. Potential species.

EBI's anti-RFP tag (RF5R) mAb has high affinity and is very flexible: it recognizes both natural and denatured forms of RFP and its various RFP variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, tdTomato, etc.

EBI's anti-GFP tag mAb (Anti-GFP Tag (GF28R) mAb) also has high affinity and is as flexible as anti-RFP tag mAb: it recognizes multiple variants of native and denatured forms of GFP and GFP: YFP , EGFP, EYFP and CFP.

EBI's anti-ERK1 signal transduction mAb (Anti-ERK1 (E32, E19) signal transduction mAbs) specifically recognizes ERK1 protein, but does not recognize ERK2 protein. These monoclonal antibodies have a particularly high affinity and are very efficient for immunoprecipitation.