At present, there are many studies on the expression mechanism and cultivation of cold water, but the effects of environmental factors on leaf color performance have not been explored. The cold water flower was treated by different light intensity, and the influence of light intensity on its leaf color change and the ability of anti-oxidation adaptation under high light stress were studied by light incubator to provide a theoretical basis for the scientific cultivation of cold water flower.
Use the top stem segment of the cold water plant as a cutting strip, cut about 15cm, keep the top 2~3 knots, and cut off the lower leaves. The substrate is sterilized and sterilized, and the substrate is placed in a small pot about 10 cm high. Two cut stalks are prepared, and the appropriate concentration of rooting powder is inserted into the substrate. The first time, the water is poured and placed at 23 ° C. Culture in a light incubator (normal light).
Different light intensity treatments in the light incubator have a great influence on the pigment content and ratio in the leaves. According to the change of CAT activity, the response of cold water flowers in the antioxidant metabolism pathway under different light intensities can be traced. Compared with normal light and shading treatment, the leaves of cold water flower leaves after glare treatment were slightly white and slightly burned. In the light incubator, the leaves are weak under dark conditions, the leaf color is not bright enough, and the Chl content is also low, second only to strong light.
The content of Ant in the leaves of cold water flower was significantly increased under the strong light induction of the light incubator, and it was the lowest under dark conditions, and it was significantly different from other treatments (P<0.05), which was the opposite of the lowest expression of Chl. The trend can initially see the negative correlation between Ant and Chl. under the same conditions. In the light incubator, the CAT activity in the leaves of cold water was increased under strong light and dark conditions, which was significantly different from normal and shading (P<0.05), indicating that CAT activity was induced under light stress.
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