After spinach germinates, the first two cotyledons grow out. The two leaves are thin, long, and swindles, looking like a grass. But after a few days, its true leaves began to sprout, and the tiny leaves just sprouted had their own shape, some with round leaves and some with pointed leaves.
When the seedlings grow two true leaves, if the seedlings in the ground are too much and too crowded, it is necessary to raise seedlings and keep the seedlings at a distance of 3 to 5 cm. The principle of thinning seedlings: Intermittent and sparse, weak and strong, inseminable and healthy. Combine with seedlings to remove field weeds and prevent weeds and seedlings from competing for nutrients. If it is drilled, it is also possible to plough loose soil after the seedlings are released. Because spinach seedlings are shallow and shallow during this period, shallow ploughing should be the main method. Before wintering, according to the field conditions, sorghum weeds two to three times. The work of sclerophyllous grasses seems simple, but it is very important for the growth of overwintering spinach. It can loosen the soil and promote the development of the leaves of spinach roots, making it grow stronger and safe for the winter. The water needed for spinach at seedling stage is not too much. If the soil is too dry, it can be poured with appropriate water, but it should not be too much. Appropriate control of water is conducive to the growth of underground roots, and it is also possible to avoid the top leaves of seedlings from sticking to sludge and causing hypoxia asphyxiation and death. After spinach grows 4 to 5 leaves, the temperature is gradually getting lower. Before the advent of winter, pouring frozen water once is good for safe wintering of spinach. The timing of pouring frozen water is very important, it is poured too early, spinach appears to be prosperous, and the dormancy period is delayed, which affects the growth of the coming year; when it is poured too late, the soil is frozen and the water accumulates at the surface and does not infiltrate, which may cause freezing damage to the spinach. .
Cell culture plates can be divided into flat bottoms and round bottoms (U-shaped and V-shaped) according to the shape of the bottom; Terasaki plates and common cell culture plates. Different shapes of culture plates serve different purposes. Cultured cells usually use a flat bottom, which is convenient for observation under the microscope, has a clear bottom area, and the height of the cell culture liquid is relatively consistent.
Different shapes of culture plates serve different purposes. Cultured cells usually use a flat bottom, which is convenient for observation under the microscope, has a clear bottom area, and the height of the cell culture liquid is relatively consistent. Therefore, when doing experiments such as MTT, whether it is adherent or suspension cells, flat bottom plates are generally used. Absorbance must be measured using a flat-bottomed culture plate. Pay special attention to the material, the label "Tissue Culture (TC) Treated" is for cell culture.
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