Taking MS medium as an example, the preparation of the mother liquid includes a large amount of elements, trace elements, iron salts, vitamins, amino acids, plant growth regulating substances and organic add-ons.
1 Preparation of a large amount of elements and trace element mother liquor
According to the prescribed dosage, we can weigh all kinds of medicines required. In order to reduce the workload during preparation, several medicines (such as large amounts of elements or trace elements) can be combined in the same mother liquor. However, due to the mixing of various drugs, a reaction may occur and a precipitate may be formed, so that the mother liquid fails. In order to avoid the occurrence of similar phenomena, in the preparation of a large amount of elements, trace elements and mother liquor of vitamins and amino acids, the following two methods can be used to solve: 1 a large amount of elements, trace elements, vitamins and amino acids, etc., which are to be combined in the same mother liquor, The drug is dissolved sequentially, after the former drug is completely dissolved, the latter chemical is added, and so on; 2 each component is completely dissolved, and then they are mixed with each other.
2 Preparation of iron salt mother liquor
The iron salt component contains ferrous sulfate (FeSO 4 ·7H2O) and disodium edetate (Na 2 -EDTA). The dissolution and mixing of these two compounds are more stringent and must be formulated as follows, otherwise precipitation will occur. The method is: separately dissolving FeSO4·7H2O and Na2-EDTA, heating and stirring constantly, completely dissolving, cooling, mixing the two solutions, adjusting the pH to 5.5, adding to the required volume with distilled water, and storing the brown bottle in In the refrigerator.
3 Preparation of plant growth regulating substance mother liquor
Plant growth regulators are one of the important components of the culture medium, generally phytohormone substances, such as auxin IAA, IBA, NAA, gibberellin GA3, cytokinin 2,4-D, KT and 6-BA, etc. Since most growth regulating substances are difficult to dissolve in water, the preparation method is also different: 1IAA, IBA and GA 3 are first dissolved with a small amount of 95% alcohol, then added with water to a constant volume, shaken, stored in a reagent bottle, labeled After storage in the refrigerator; 2NAA can be dissolved with hot water or a small amount of 95% alcohol, and then added to the required volume with water; 32,4-D can be dissolved in a little 1mol / L NaOH solution, then add water to volume; 4KT And 6-BA can be dissolved in a small amount of 1mol / L HCl, and then added to volume of water; 5 zeatin is first dissolved in a small amount of 95% alcohol, and then added to volume with water.
The concentration of plant growth regulators is usually expressed in ppm (mg/ml) and mol/L. It is often convenient to use ppm in the preparation of the mother liquor. It is usually formulated into a mother liquor of 0.5 ppm. This concentration is convenient for calculation and avoids formation during refrigeration. crystallization.
4 Preparation of organic additive mother liquor
In plant tissue culture, some organic addenda, such as coconut juice, is often added to the culture medium to promote tissue culture activity. Since the active ingredient of coconut juice is heat-resistant, in the preparation, the juice collected from the fruit is first heated and boiled to remove the protein, filtered, and then stored in a plastic bottle at a low temperature refrigerator at -20 ° C. .
There are so many precautions for arranging the medium. For quick and easy, you can buy ready-made medium dry powder.
Ms medium http://?kw2=ms%C5%E0%D1%F8%BB%F9&tn=5
B5 medium http://
N6 medium http://
Herb extract we mainly supply are monk fruit extract, shiitake mushroom extract, reshi mushroom extract and other mushroom extract like lion`s mane extract. The main active ingredient among those powders are polysaccharides, beta glucan and so on. And monk fruit extract is one of our hot selling natural sweetener which can be added in very wide range of food and beverage. Unlike sugar, it is a kind of non- nutritive sweetener which is low in calories and high in sweetness, welcome to reach us for more information.
Herb Extract,Reishi Mushroom Extract,Mushroom Extract,Shiitake Mushroom Extract
YT(Xi'an) Biochem Co., Ltd. , https://www.ytwholefood.com