1. Cultivation season: seedlings can be planted from mid-June to mid-July, and planted in greenhouses from mid-July to mid-August, and harvested in early October.
2. Seeding and seedling: Before planting, the nursery bed should be properly watered, and covered with 1 cm thick sieved soil after sowing. Generally, 3 to 5 grams of seedlings per square meter are used, and a planting area of ​​6 to 8 square meters per acre is required. The ground moisture of the seedling bed before emergence should be controlled at 20 ° C during the day and 12 ° C to 15 ° C at night. When the seedlings grow 2 true leaves, the seedlings are divided into evening or cloudy days, and the row spacing is about 10 cm and 10 cm. After planting, the water is irrigated and the temperature is controlled between 25 ° C and 28 ° C during the day and 15 ° C to 18 ° C during the night. The temperature after slow seedling control is 20 ° C ~ 25 ° C during the day, 13 ° C ~ 15 ° C at night. In summer, due to high temperatures, water should be watered frequently. When the seedlings grow to 8 leaves, they are colonized.
3. Colonization: Select sandy loam with deep soil, fertile soil, good ventilation, convenient drainage, neutral pH or slightly acidic for colonization. 7 to 10 days before planting, the plant spacing of single-stalk pruning is 25-30 cm, and the spacing of double-stalk pruning is 45-50 cm. 80-100 kg of quicklime, 2000 kg of decomposed organic fertilizer, 80 kg of cake fertilizer, 30 kg of superphosphate and 40 kg of compound fertilizer per acre. Among them, the cake fertilizer and 60% organic compost are sprinkled into the soil before the last land preparation, and the remaining mixture is fully mixed and applied to the colonization hole.
4. Field management: After the first spike flower is placed, the fruit is poured once. During the result, water is poured every 5 to 6 days, and the soil is required to see dry and wet, and the watering period is reduced to prevent cracking. After slow seedling to flowering, apply 10 to 15 kilograms of quick-acting compound fertilizer per acre. After the first ear of fruit is hung, it will be chased once again to preserve the fruit fertilizer. Afterwards, the fruit is harvested in batches to supplement the topdressing 2 to 3 times. The fertilizer for the cherry tomato in the fruiting period should be applied with manure or special compound fertilizer, and the general compound fertilizer for acre is about 10 kg. At the peak of the season, 0.3% potassium dihydrogen phosphate can be sprayed on the foliage.
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Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
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