First, a fixed number, less feed Tim. From the 30-day-old piglet, it is necessary to increase the number of feeds, generally feeding 5-6 times a day, and a fixed time, so that pigs develop the habit of regular feeding. The piglet feeds regularly to make it full and hungry. It can increase digestion and maintain appetite without affecting the feed intake of the next meal. Each feeding must also be fed less frequently, which not only meets the piglet's grazing habits, but also adapts to the characteristics of piglets that love to eat fresh feed. Every time fresh feed is added, the piglets compete for food and eat a little more feed in the invisible room. At the same time, less feeding, can reduce feed wastage.
Second, to vote for it, support the belly. Use piggers to eat more feeds in the evening, first feed the general feed, and then mix some fishmeal and other feeds to eat more. When the piglet is about to feed, feed the feed that the pig prefers, such as grain corn, sprouted barley, Melons and so on. Finally feed green and give clean water. As a result, the belly of piglets will grow bigger and their appetite will increase.
Third, stabilize the feed and grab a sizzling meal. Pigs must have stable feeds during the vigorous food phase and do not change too much. Because once the piglets get used to eating some kind of feed, sudden changes often affect the number of feed intake, and they do not even eat, and it takes a few days to get used to it. If there are changes, the appetite will be even more affected. The so-called “feed instability, Wangwangfeijin†is the reason. The piglets are in a hurry, winter is in the afternoon and summer is in the evening. During this time, the feed intake of piglets can account for more than half of the feed for the whole day, which is the prime time for the piglets to feed.
Fourth, increase protein feed, add trace elements. Piglets grow rapidly during the affluent period, and their absorption of protein is also very strong. Therefore, attention should be paid to supplement protein feed and trace elements, can feed more bean cakes, bean dregs, fish meal or small fish, shrimp, etc., generally do not occur diarrhea, and can promote Wang Shi, so that piglets grow faster.
The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
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